Fig. 5

Analysis of subcellular localization for ZmWAKL proteins and TFs. A Subcellular localization analysis conducted in N. benthamiana leaves. NLS-RFP were used as nuclear marker and show red fluorescence signals. PIP2-cherry was exclusively employed as a cell membrane marker in conjunction with ZmWAKL52-GFP co-transformation in tobacco leaves, manifesting a distinctive red fluorescent signal. Scale bar = 25 µm. B Subcellular localization examination of ZmWAKL52 in onion epidermal cells following plasmolysis. The arrow indicates GFP signals present in cell wall. Scale bar = 100 µm