Fig. 1

Phylogenetic analysis of P-type ATPases from A. thaliana, H. vulgare, and O. sativa. Full-length protein sequences were aligned in MEGA6 using MUSCLE with standard parameters. The alignment was then subjected to maximum likelihood analysis and Bayesian inference. For maximum likelihood analysis, the RAxML-HPC2 workflow implemented in the XSEDE tool was used on the CIPRES Science Gateway with the following parameters: bootstraps = 1000; data type = protein; Protein Substitution Matrix = LG; and the rest of the settings were default. For Bayesian inference analysis, the MrBayes v. 3.2.7 tool was used on the CIPRES Science Gateway with the following parameters: aamodelpr = fixed(lg); prset statefreqpr = fixed(empirical); lset rates = invgamma; mcmcp ngen = 100,000; mcmcp nruns = 2; mcmcp nchains = 8; mcmcp temp = 0.05; mcmcp mcmcdiagn = yes; and mcmc diagnfreq = 10,000. Numbers at nodes show likelihood from maximum likelihood analysis, and filled circles indicate full support in Bayesian inference analysis. In the phylogenetic analysis, the orthologue of OsALA6 was excluded as this gene is partial