Table 3 Abundance of genes in transcript per million (TPM) involved in structural constituent of cuticle and microtubule-based process in the transcriptomes of C. sapidus ovigerous setae. Transcriptomes: prepuberty females at early premolt (OE) and late premolt (OL), and adults (AO). |log2 (fold change)| < 1 is noted by ‘-’ symbols. Expression was also determined using genome mapping of the RNAseq data in the last three columns
Seq ID | Gene Name | Gene Abbreviation | C. sapidus Genome Location | Top NCBI Accession NO. | Trinity TPM Value | Trinity Log2 (fold change) Value | Genome mapping TPM value | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
OE | OL | AO | OE vs. OL | OL vs. AO | OE vs. AO | OE | OL | AO | |||||||||
Structural constituent of cuticle: chitin_bind_4 domain with RR-1 sequence | Â | Â | Â | ||||||||||||||
TRINITY_DN9_c0_g1 | arthrodial membrane protein AMP1A-like | AMP1A-like | HiC_scaffold_20: 23834608.. 24379310 | XP_045136044.1 | 2.24 | 4,372.78 | 2.17 | 12.73 | -9.33 | - | 0 | 3,497.74 | 0.17 | ||||
TRINITY_DN333_c4_g1 | arthrodial membrane protein AMP8.1 | AMP8.1 | HiC_scaffold_16: 5226063..5227150 | AAV28476.1 | 6.97 | 1,506.03 | 301.52 | 6.40 | -2.70 | 5.43 | 4.85 | 1,071.43 | 312.86 | ||||
TRINITY_DN44_c1_g2 | larval cuticle protein LCP17-like | LCP17-like | HiC_scaffold_16: 3902805..3903928 | XP_045101992.1 | 11.09 | 525.71 | 22.22 | 4.23 | -4.94 | - | 8.52 | 450.78 | 29.98 | ||||
TRINITY_DN86_c2_g1 | calcified cuticle protein CP8.5 | CP8.5 | HiC_scaffold_26: 11503353..11504081 | AAV28478.1 | 0.14 | 238.01 | 0.08 | 9.37 | -6.16 | - | 0 | 245.13 | 0 | ||||
Chitin_bind_4 domain with RR-2 sequence | Â | Â | Â | ||||||||||||||
TRINITY_DN11_c0_g1 | cuticle protein 7-like | CP7-like | HiC_scaffold_35: 1259650..2443401 | XP_045107367.1 | 1.37 | 14,684.52 | 0.74 | 12.50 | -15.12 | - | 0.88 | 15,067.56 | 1.06 | ||||
TRINITY_DN1246_c1_g1 | adult-specific cuticle protein ACP20 | ACP20 | HiC_scaffold_11024: 1191..1640 | XP_045101342.1 | 0.47 | 3,109.80 | 1.53 | 11.35 | -5.72 | 1.70 | 0.57 | 4,269.25 | 0.67 | ||||
TRINITY_DN38_c23_g2 | pro-resilin | Pro-resilin | HiC_scaffold_27: 4189445..4189982 | XP_042211318.1 | 0.12 | 991.54 | 0.04 | 11.64 | -14.94 | -1.58 | 0.27 | 1149.54 | 0.068 | ||||
TRINITY_DN2304_c0_g1 | cuticle protein 6 | CP6 | HiC_scaffold_20: 24226901..24228133 | MPC43157.1 | 0.12 | 455.13 | 0.40 | 10.31 | -4.86 | 1.74 | 0.11 | 167.07 | 0.546 | ||||
TRINITY_DN181_c0_g1 | cuticle protein 8-like | CP8-like | HiC_scaffold_28: 10632703..10633852 | XP_045101387.1 | 0.03 | 111.22 | 0.05 | 10.31 | -5.68 | - | 0 | 97.30 | 0.06 | ||||
Chitin_bind_4 domain without RR sequence | Â | Â | Â | ||||||||||||||
TRINITY_DN5111_c0_g1 | calcified cuticle protein CP15.0 | CP15.0 | HiC_scaffold_37: 4750563..4751141 | ABB91679.1 | 0.15 | 114.43 | 0.01 | 8.16 | -8.88 | -3.91 | 0 | 95.48 | 0 | ||||
Microtubule-based process | Â | Â | Â | ||||||||||||||
TRINITY_DN19_c0_g1 | tubulin alpha-1 chain | TUBA1 | HiC_scaffold_15: 1506280..1511624 | XP_045137688.1 | 1,214.19 | 2,233.57 | 780.17 | - | -1.90 | - | Â | Â | Â | ||||
TRINITY_DN197_c34_g1 | tubulin beta-1 chain | TUBB1 | HiC_scaffold_33: 21557045..21562537 | XP_045129538.1 | 1,076.71 | 946.54 | 411.23 | - | -1.10 | -1.54 | Â | Â | Â | ||||