Fig. 2

Relative expression level of Mg-odr-7, Mg-tax-4, Mg-tax-4.1, Mg-osm-9 and Mg-ocr-2 mRNAs in different developmental stages of M. graminicola. Fold change in target gene expression was set as 1 in adult female, and statistically compared with expression in other developmental stages including egg, pre- and post-parasitic J2, J3/J4, young and adult egg-laying female. Each bar represents mean fold change value of qPCR runs in three biological and technical replicates ± standard errors. Bars with different letters indicate significant difference according to the Tukey’s HSD test, P < 0.01. Gene expression was normalized using two housekeeping genes of M. graminicola (actin and 18 S rRNA).