Fig. 4

Uptake of dsRNA molecules inside nematode body was visually assessed. DsRNA molecules were labeled with a tracking dye Alexa Fluor 488. Fluorescence imaging showed that dsRNA molecules corresponding to Mg-odr-7, Mg-tax-4, Mg-tax-4.1, Mg-osm-9 and Mg-ocr-2 genes were effectively ingested by M. graminicola J2 after overnight soaking. Soaking buffer treated worms indicated no fluorescence (slight greenish appearance maybe because of the autofluorescence). Scale bar = 50 μm