Fig. 5

Target-specific downregulation of Mg-odr-7, Mg-tax-4, Mg-tax-4.1, Mg-osm-9 and Mg-ocr-2 mRNAs in J2s treated with corresponding dsRNAs at 24 h post soaking. Nematodes treated with gfp dsRNA and soaking buffer were used as the non-native and negative control, respectively. M. graminicola actin and 18 S rRNA genes were used to normalize the fold change in expression data. Each bar represents mean fold change value of qPCR runs in three biological and technical replicates ± standard errors. Fold change in target gene expression was set as 1 in soaking buffer-treated worms, and statistically compared with other treatments. Bars with different letters indicate significant difference according to the Tukey’s HSD test, P < 0.01