Table 1 Number of contigs passing the 1.5 coverage ratio threshold after the genomic mappings performed trying to reproduce the results in [1]. We first used the A. mexicanus transcriptome as reference to estimate 1B/0B coverage ratio for the contigs claimed by authors as being in the B chromosome of A. correntinus (61 and 26 contigs, the sequence of which we downloaded from EBI and NCBI, respectively). In different mappings, we expressed the results as “number of reads mapped” (as done in [1]) or in “number of copies per haploid genome” (as done in [2]). According to [1], all 61 and 26 contigs passed the 1.5 coverage ratio threshold. However, note the low figures of reproducibility in all our attempts, from using [1] reads as such, normalizing the results for 0B and 1B library size differences, or using equal number of reads from each library. In addition, our analysis using the transcriptome from two species (A. scabripinnis and A. paranae) being closely related to A. correntinus, yielded 630 contigs passing the 1.5 threshold but only one (corresponding to the ipo11 gene) was also included in the 61 and 26 contigs considered B-genes in [1]

From: Irreproducible results and unsupported conclusions in Ahmad et al. [BMC genomics (2020) 21:656]