Fig. 1

The phylogenetic relationships and conserved domain analysis of CsCOL proteins in Cymbidium sinense with COL proteins from Arabidopsis thaliana (At), Oryza sativa (Os), Zea mays (Zm) and Hordeum vulgare (Hv). The tree is displayed as a phylogram in which branch lengths are proportional to distance. Bootstrap values for 1000 replicates were used to assess the robustness of the trees. The domain structure of B-box 1 (red rectangles), B-box 2 (purple rectangles), second divergent B-box 2 (blue rectangles) and CTT (dark blue rectangles) of the COL amino acid sequences are shown on the right side. The proteins used for alignment are as follows: A. thaliana AtCO (AT5G15840.1), AtCOL1(AT5G15850.1), AtCOL2 (AT3G02380.1), AtCOL3 (AT2G24790.1), AtCOL4 (AT5G24930.1), AtCOL5 (AT5G57660.1), AtCOL6 (AT1G68520.1), AtCOL7 (AT1G73870.1), AtCOL8 (AT1G49130.1), AtCOL9 (AT3G07650.1), AtCOL10 (AT5G48250.1). AtCOL11 (AT4G15250.1), AtCOL12 (AT3G21880.1), AtCOL13 (AT2G47890.1), AtCOL14 (AT2G33500.1), AtCOL15 (AT1G28050.1), AtCOL16 (AT1G25440.1); O. sativa OsHd1 (NM_001421538.1), OsCOL3 (NM_015770369.2), OsCOL5 (XM_015785723.2), OsCOL9 (XM_026022873.1), OsCOL16 (XM_015767173.2); Z. mays ZmaCOL3 (GRMZM2G012717_P01), ZmaCOL7 (GRMZM2G041991_P01), ZmaCOL8 (GRMZM2G095598_P01), ZmaCOL12 (GRMZM2G042198_P01), ZmaCOL16 (GRMZM2G013398_P04); H. vulgare HvCO2 (AAM74064.1), HvCO3 (AAM74068.1), HvCO4 (AAM74069.1), HvCO6 (AAL99268.1), HvCO8 (AAL99270.1)