A comprehensive assessment of exome capture methods for RNA sequencing of formalin-fixed and paraffin-embedded samples

Table 2 The alignment statistics to the human genome

No.	Library	Genome Mapping Rate (%)	Gene Mapping Rate (%)	Sense Rate (%)	Genes detected number	Transcripts detected number
1	FFPE_RD_1	98.07	32.56	96.27	16,077	53,547
2	UHRR_RD_1	97.64	40.52	96.26	17,157	59,524
3	FFPE_RD_2	97.57	33.46	95.87	16,108	53,972
4	UHRR_RD_2	97.77	40.53	96.24	17,158	60,039
5	FFPE_IL_1	95.52	79.47	99.51	15,467	47,185
6	UHRR_IL_1	92.12	76.89	99.39	16,420	48,113
7	FFPE_IL_2	95.03	79.13	99.52	15,530	47,692
8	UHRR_IL_2	94.24	77.03	99.42	16,374	47,190
9	FFPE_AG_1	96.01	73.71	97.38	16,683	55,486
10	UHRR_AG_1	97.56	75.76	97.54	17,196	59,114
11	FFPE_AG_2	97.15	73.7	96.71	16,655	53,422
12	UHRR_AG_2	96.8	73.83	97.37	17,038	56,474
13	FFPE_NS_1	92.71	74.12	64.12	17,410	59,360
14	UHRR_NS_1	91.03	70.31	68.08	17,799	60,125
15	FFPE_NS_2	92.77	73.64	70.36	17,595	59,859
16	UHRR_NS_2	91.54	71.57	71.48	17,865	60,590
17	FFPE_NP_1	95.93	75.48	97.99	16,885	57,648
18	UHRR_NP_1	96.34	76.48	98.22	17,303	60,355
19	FFPE_NP_2	95.66	73.33	98.08	16,699	54,446
20	UHRR_NP_2	96.05	76	98.35	17,136	57,652

ISSN: 1471-2164