Fig. 4

Fluorescence images showing the green fluorescence (GFP) expressing cells, GluIIß KO A549 cells and non-target transfected cells, surrounded by Jurkat E6.1 T cells in the co-culture experiment (A). Viability and proliferation of Jurkat E6.1 T cells after being exposed to GluIIß KO A549 cells or non-target control cells for 24 hours as measured at 0, 12, 24, 48 and 72 hours using alamarBlue^® (B). Cancer cell lysing activity of Jurkat E6.1 T cells and PBMCs co-cultured with GluIIß KO A549 cells compared to those co-cultured with non-target transfected cells (C). Bar graphs represent means and standard deviations (SDs) from three independent experiments. *Significant different from those co-cultured with non-target transfected cells (p<0.05 by Mann Whitney U test)