Table 6 Pathways in A549 lung adenocarcinoma cells significantly downregulated by GluIIß knockout as identified by the Reactome enrichmenr analysis

1

R-HSA-1474244

Extracellular matrix organization

1.90E-18

COL5A1/ADAM19/TNC/SERPINE1/NID2/LTBP2/ITGB3/COL13A1/FBN1/FBN2/VCAN/

BMP2/COL4A1/COL4A6/ITGA4/COL8A1/COL20A1/MMP1/LOXL2/COL6A2/MMP10/

LAMC2/EFEMP1/COL7A1/LAMA4/LUM/TIMP2/MMP19/CTSB/COL16A1/COL6A1/

MMP2/NTN4/TGFB2/TLL1/COL4A2/CTSS/ADAMTS16/ITGA2/MMP17/COL4A5/P4HA3/

ICAM1/COL17A1/COLGALT2/DMD/THBS1/ITGA5/SDC4/MFAP5/ADAMTS14/PLOD2/

ADAM12/ITGB6/FBLN2/FGF2/TGFB1/COL28A1/CD44/COL1A1

60

2

R-HSA-216083

Integrin cell surface interactions

4.70E-09

COL5A1/TNC/ITGB3/COL13A1/FBN1/COL4A1/COL4A6/ITGA4/COL8A1/COL6A2/

COL7A1/LUM/COL16A1/COL6A1/COL4A2/ITGA2/COL4A5/ICAM1/THBS1/ITGA5/

ITGB6/CD44/COL1A1

23

3

R-HSA-1474290

Collagen formation

6.76E-09

COL5A1/COL13A1/COL4A1/COL4A6/COL8A1/COL20A1/LOXL2/COL6A2/LAMC2/

COL7A1/CTSB/COL16A1/COL6A1/TLL1/COL4A2/CTSS/COL4A5/P4HA3/COL17A1/

COLGALT2/ADAMTS14/PLOD2/COL28A1/COL1A1

24

4

R-HSA-1650814

Collagen biosynthesis and modifying enzymes

1.56E-08

COL5A1/COL13A1/COL4A1/COL4A6/COL8A1/COL20A1/COL6A2/COL7A1/COL16A1/

COL6A1/TLL1/COL4A2/COL4A5/P4HA3/COL17A1/COLGALT2/ADAMTS14/PLOD2/

COL28A1/COL1A1

20

5

R-HSA-1474228

Degradation of the extracellular matrix

3.48E-08

COL5A1/COL13A1/FBN1/FBN2/COL4A1/COL4A6/COL8A1/MMP1/COL6A2/MMP10/

LAMC2/COL7A1/TIMP2/MMP19/CTSB/COL16A1/COL6A1/MMP2/TLL1/COL4A2/

CTSS/ADAMTS16/MMP17/COL4A5/COL17A1/CD44/COL1A1

27

6

R-HSA-1442490

Collagen degradation

6.34E-08

COL5A1/COL13A1/COL4A1/COL4A6/COL8A1/MMP1/COL6A2/MMP10/COL7A1/

MMP19/CTSB/COL16A1/COL6A1/MMP2/COL4A2/COL4A5/COL17A1/COL1A1

18

7

R-HSA-8948216

Collagen chain trimerization

7.58E-08

COL5A1/COL13A1/COL4A1/COL4A6/COL8A1/COL20A1/COL6A2/COL7A1/

COL16A1/COL6A1/COL4A2/COL4A5/COL17A1/COL28A1/COL1A1

15

8

R-HSA-6783783

Interleukin-10 signaling

1.88E-07

CXCL1/CXCL8/IL6/CSF3/CSF2/CCL20/CXCL2/PTGS2/CCL5/IL1B/CCL2/IL1A/

ICAM1/PTAFR

14

9

R-HSA-3000178

ECM proteoglycans

5.85E-07

COL5A1/TNC/SERPINE1/ITGB3/VCAN/COL4A1/COL4A6/COL6A2/LAMA4/LUM/

COL6A1/TGFB2/COL4A2/ITGA2/COL4A5/ITGB6/TGFB1/COL1A1

18

10

R-HSA-3000171

Non-integrin membrane-ECM interactions

1.54E-06

COL5A1/TNC/ITGB3/COL4A1/COL4A6/LAMC2/LAMA4/NTN4/COL4A2/ITGA2/

COL4A5/DMD/THBS1/SDC4/FGF2/TGFB1/COL1A1

17

11

R-HSA-2022090

Assembly of collagen fibrils and other multimeric structures

5.46E-06

COL5A1/COL4A1/COL4A6/COL8A1/LOXL2/COL6A2/LAMC2/COL7A1/CTSB/

COL6A1/TLL1/COL4A2/CTSS/COL4A5/COL17A1/COL1A1

16

12

R-HSA-1566948

Elastic fibre formation

1.45E-05

LTBP2/ITGB3/FBN1/FBN2/BMP2/LOXL2/EFEMP1/TGFB2/ITGA5/MFAP5/ITGB6/

FBLN2/TGFB1

13

13

R-HSA-381426

Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)

3.58E-05

TNC/IGFBP7/C3/EVA1A/IL6/FSTL1/CDH2/PRKCSH/FBN1/VCAN/MFGE8/MMP1/

PAPPA/IGFBP6/MMP2/APOL1/VGF/CYR61/TMEM132A/NOTUM/MSLN

21

14

R-HSA-2214320

Anchoring fibril formation

4.10E-05

COL4A1/COL4A6/LAMC2/COL7A1/TLL1/COL4A2/COL4A5/COL1A1

8

15

R-HSA-2129379

Molecules associated with elastic fibres

7.17E-05

LTBP2/ITGB3/FBN1/FBN2/BMP2/EFEMP1/TGFB2/MFAP5/ITGB6/FBLN2/TGFB1

11

16

R-HSA-6785807

Interleukin-4 and 13 signaling

0.000406901

CXCL8/IL6/SOX2/SOCS3/PTGS2/SAA1/MMP1/LCN2/IL1B/FSCN1/MMP2/CCL2/

IL1A/ICAM1/TWIST1/MUC1/FGF2/TGFB1

18

17

R-HSA-3000170

Syndecan interactions

0.000797158

COL5A1/TNC/ITGB3/ITGA2/THBS1/SDC4/FGF2/TGFB1/COL1A1

9

18

R-HSA-375276

Peptide ligand-binding receptors

0.000892778

CXCL5/C3/CXCL1/CXCL8/CXCL3/CCL20/CXCL2/NPY4R2/LOC105379861/

SAA1/EDN2/NTSR1/CCL5/F2RL1/F2R/NPY4R/PPBP/C5AR2

18

19

R-HSA-500792

GPCR ligand binding

0.001395839

CXCL5/C3/GNG2/CXCL1/CXCL8/DRD1/CXCL3/CCL20/CXCL2/NPY4R2/

LOC105379861/SAA1/EDN2/NTSR1/WNT5A/CCL5/UCN2/F2RL1/F2R/

NPY4R/ADRB2/HRH1/GIP/ADGRE1/ADRB1/FZD2/PTAFR/CD55/PPBP/C5AR2/GNGT2/S1PR3

32

20

R-HSA-380108

Chemokine receptors bind chemokines

0.001395839

CXCL5/CXCL1/CXCL8/CXCL3/CCL20/CXCL2/CCL5/PPBP

8

21

R-HSA-8957275

Post-translational protein phosphorylation

0.001395839

TNC/IGFBP7/C3/EVA1A/IL6/FSTL1/CDH2/PRKCSH/FBN1/VCAN/MFGE8/

APOL1/VGF/CYR61/TMEM132A/NOTUM/MSLN

17

22

R-HSA-3000157

Laminin interactions

0.001599807

NID2/COL4A1/COL4A6/LAMC2/COL7A1/LAMA4/COL4A2/ITGA2/COL4A5

9

23

R-HSA-2243919

Crosslinking of collagen fibrils

0.001704251

COL4A1/COL4A6/LOXL2/TLL1/COL4A2/COL4A5/COL1A1

7

24

R-HSA-373076

Class A/1 (Rhodopsin-like receptors)

0.001803729

CXCL5/C3/CXCL1/CXCL8/DRD1/CXCL3/CCL20/CXCL2/NPY4R2/LOC105379861/

SAA1/EDN2/NTSR1/CCL5/F2RL1/F2R/NPY4R/ADRB2/HRH1/ADRB1/PTAFR/PPBP

/C5AR2/S1PR3

24

25

R-HSA-2173782

Binding and Uptake of Ligands by Scavenger Receptors

0.002478535

JCHAIN/COL4A1/SAA1/SCARA5/APOL1/COL4A2/LRP1/SCARF1/COL1A1

9

26

R-HSA-5173105

O-linked glycosylation

0.003109464

GALNT5/GALNT16/GALNT9/ADAMTSL4/ADAMTSL1/ADAMTS16/SPON2/GALNT6/

ADAMTS12/ADAMTS6/THBS1/B3GNT7/B3GNT5/ADAMTS14/MUC3A/MUC1

16

27

R-HSA-449147

Signaling by Interleukins

0.003267263

IL11/CXCL1/IL31RA/CXCL8/IL6/CSF3/CSF2/SOX2/CCL20/IL32/CXCL2/IL33/IL7R/

SOCS3/SOD2/PTGS2/SAA1/EBI3/MMP1/LCN2/IRAK2/CCL5/IL1B/STAT5A/FSCN1/

MMP2/PIK3CD/CCL2/IL1A/SOCS2/CRLF2/ICAM1/IL24/JUN/TWIST1/CSF2RA/

PTAFR/RPS6KA5/MUC1/FGF2/IL22RA2/TGFB1

42

28

R-HSA-419037

NCAM1 interactions

0.007391944

COL5A1/COL4A1/COL6A2/GFRA1/COL6A1/COL4A2/GFRA2/COL4A5/GDNF

9

29

R-HSA-75205

Dissolution of Fibrin Clot

0.007391944

SERPINE1/PLAU/PLAT/SERPINE2/PLAUR

5

30

R-HSA-5083635

Defective B3GALTL causes Peters-plus syndrome (PpS)

0.007829636

ADAMTSL4/ADAMTSL1/ADAMTS16/SPON2/ADAMTS12/ADAMTS6/THBS1/ADAMTS14

8

31

R-HSA-418990

Adherens junctions interactions

0.009382596

CDH11/CDH4/CDH2/CDH12/CDH8/CDH6/CADM1/NECTIN3

8

32

R-HSA-5173214

O-glycosylation of TSR domain-containing proteins

0.009382596

ADAMTSL4/ADAMTSL1/ADAMTS16/SPON2/ADAMTS12/ADAMTS6/THBS1/ADAMTS14

8

33

R-HSA-186797

Signaling by PDGF

0.021385068

COL5A1/PLAT/COL4A1/COL6A2/STAT5A/COL6A1/COL4A2/COL4A5/THBS1/PDGFRA

10

34

R-HSA-3906995

Diseases associated with O-glycosylation of proteins

0.021385068

ADAMTSL4/ADAMTSL1/ADAMTS16/SPON2/ADAMTS12/ADAMTS6/THBS1/

ADAMTS14/MUC3A/MUC1

10

35

R-HSA-375165

NCAM signaling for neurite out-growth

0.041925906

COL5A1/COL4A1/COL6A2/GFRA1/COL6A1/COL4A2/GFRA2/COL4A5/RPS6KA5/GDNF

10

36

R-HSA-3928665

EPH-ephrin mediated repulsion of cells

0.041925906

EPHA5/EPHB2/EFNA1/CLTCL1/EPHA4/MMP2/EFNB2/EFNB3/EFNA3

9

37

R-HSA-5686938

Regulation of TLR by endogenous ligand

0.041925906

S100A9/TLR4/SFTPD/S100A8/CD14

5

38

R-HSA-2980736

Peptide hormone metabolism

0.041925906

ANPEP/PCSK1/INHBA/PAX6/CGB8/ENPEP/GIP/CGB5/KIF5A/ACHE/MYRIP

11