Fig. 2

WGS depth has a limited effect on fusion transcript validation rate. A Reads from breast tumors with high-coverage WGS were subsampled to 0.25, 0.5 and 0.75 of the original coverage and used for validation. Most validated fusions are still detected at a low coverage of 13x. B Fraction of fusion transcripts validated per sample plotted against WGS sequencing depth showing a weak positive correlation. C Compared to cancer type, sequencing depth has a negligible effect on the fraction of validated fusion transcripts in linear modeling