Fig. 5

Genomic breakpoints provide information about mechanisms creating gene fusions. A Microhomology between fusion partners was significantly more common at genomic breakpoints than in control regions. B Nucleotide positions flanking genomic breakpoints had higher sequencing depth in WGS data relative to the genome average with a distinct drop on the side of the breakpoint that was not in the fusion transcript. C The higher relative sequencing depth was especially pronounced in intrachromosomal fusions, indicating amplification as a possible mechanism. D Exons included in validated fusion transcripts (n=1295) had higher expression than excluded exons of the same gene and sample in the SCAN-B cohort where exon-level expression was available