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Fig. 6 | BMC Genomics

Fig. 6

From: Comprehensive genome‑wide analysis of the chicken heat shock protein family: identification, genomic organization, and expression profiles in indigenous chicken with highly pathogenic avian influenza infection

Fig. 6

Validation of nine differentially expressed chicken HSP genes. The two indigenous Ri chicken lines were infected with 104 EID50 of HPAIV. Lung tissues were isolated on post-infection days 1 and 3, and the transcript levels of indicated genes were determined by reverse transcription followed by quantitative PCR (RT-qPCR). Gene expression levels were normalized to those of GAPDH. Experiments were performed in triplicates on samples from five chickens. Significant differences in mRNA expression levels between two chicken lines are indicated as follows: *, p < 0.05; **, p < 0.01; and ***, p < 0.001. Error bars indicate SE of technical replicates (triplicates)

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