Comprehensive genome‑wide analysis of the chicken heat shock protein family: identification, genomic organization, and expression profiles in indigenous chicken with highly pathogenic avian influenza infection

Table 2 Primers used for quantitative PCR (qPCR) validations

Primer	F/R	The nucleotide sequence (5′-3′)	Accession No	Product Length (bp)
GAPDH	F	TGC TGC CCA GAA CAT CAT CC	NM_204305
GAPDH	R	ACG GCA GGT CAG GTC AAC AA	NM_204305
DNAJB11	F	GGC CGC GTC TGT CTT CTC T	XM_422682	118
DNAJB11	R	GGT ACG CCT TCT TGA TGT CCT T	XM_422682	118
DNAJB13	F	CCT CAC GCC CGA CAA GAA	XM_417251	117
DNAJB13	R	AAT ACC GGG GAA CGC TCA CT	XM_417251	117
DNAJC5	F	ACG TGC TGG GAC TGG ACA AG	NM_001278008	114
DNAJC5	R	TTT CTG CCG CCT CTG GAT TA	NM_001278008	114
DNAJC13	F	TGC TGG GCT CAA GGT ATG G	XM_004939445	115
DNAJC13	R	TAA GCG TAG CAA GGT CAG TCT C	XM_004939445	115
DNAJC14	F	ACA GGC AGG CAG CAG AGG AGT	XM_001232018	127
DNAJC14	R	CAG GGG GAG GTG GGA AGC	XM_001232018	127
DNAJC21	F	TCT CTA CTG CCC TGC TTG TGA C	XM_004937181	83
DNAJC21	R	TCT CCC GAT GCT TCT TTG A	XM_004937181	83
HSP7	F	CTG CCC GAG GAT GTG GAC	XM_427836	105
HSP7	R	GAC GTG CTC GCT GTG CTT AG	XM_427836	105
HSP10	F	GCG CAG CAG AGA CCG TAA C	AF031309	97
HSP10	R	GCT CCC GAT CCA ACT GCT A	AF031309	97
HSPA2	F	GGC CTT CAC CGA TAC AGA GC	NM_001006685	109
HSPA2	R	ATA CTT GCG GCC GAT GAG AC	NM_001006685	109

ISSN: 1471-2164