Fig. 4

Visualization of the pooled effect sizes of the sample parameters on quality metrics, acquired from the multiplicative GLMs. Abbreviations for the sample parameters are: error rate (ERR), sequencing depth (SEQD), PCR duplicate ratio (PCRD), and optical duplicate rate (OPTD). Where sample parameters are connected with “ × ” represent the (pooled) interaction coefficients from the models. NS and (S) label non-significant (P > 0.05) and suggestive (0.003 < P < 0.05) association, whereas the absence of labels indicate significant (P < 0.003) effect. Blue and red colors in the tiles’ background indicate negative and positive pooled effect sizes, respectively. In addition, filled circles within tiles represent homogeneity measures from the meta-analytical models; specifically, their size is proportional to the meta-analytic models' between-reference-genome heterogeneity quantified by I², whereas color represents the heterogeneity variance τ.² (from grey to light green for small to larger values, respectively)