Fig. 1
From: MAGICIAN: MAG simulation for investigating criteria for bioinformatic analysis
A Diagram of the process in the simulated experiments; 1: the user supplies genomes and their required relative abundances for one or more community samples. 2: Microbiomes with the user-supplied compositions are simulated. 3: Shotgun metagenomic sequencing of the microbiomes is simulated resulting in reads with the chosen error profile. 4: Metagenomic assembly is performed on each microbiome. 5: Genomic binning is performed in an attemt to group the contigs/scaffolds into original input genomes. 6: The metagenomic bins are compared to the user-supplied input genomes. 7: Summary tables are provided to the user showing matched pairs of genomes and bins, how well they cover each other and differ in terms of fragmentation, % GC, length, and completion. Note that only a single summary table corresponding to community_1 (left) is shown. B A detailed overview of the workflow of MAGICIAN, from generation of configuration files for CAMISIM to summarizing quality control measures, showing the software used. Created with BioRender.com