Fig. 8

Expression analysis and plate stress validation of GeRAV1 in Escherichia coli Rosetta (DE3) cells. (A) Induced expression of GeRAV1 in E. coli Rosetta (DE3) cells. M: Protein marker; 1: blank control (uninduced E. coli Rosetta (DE3) cells); 2: blank control induced for 12 h; 3: control group (uninduced pET32a-Rosetta strain); 4: control group induced for 12 h; 5: uninduced recombinant strain (pET32a-GeRAV1-Rosetta strain); 6–10: recombinant strain induced at 37°C for 2, 4, 6, 8, and 12 h, respectively; 11–12: recombinant strain induced at 16°C for 2 and 12 h, respectively; 13–14: recombinant strain induced at 28°C for 2 and 12 h, respectively. The red boxes indicated the induced target protein. (B) Spot assay to verify the growth ability of pET32a-Rosetta cells and pET32a-GeRAV1-Rosetta cells on LB plates under 4°C, NaCl (sodium chloride), and mannitol stresses