Fig. 1
From: Functional annotation of a divergent genome using sequence and structure-based similarity
Functional annotation of V. necatrix genes using structure-prediction and sequence-based comparative analyses. a) A phylogenetic tree based on [25] with 24 microsporidian species, and 3 outgroup species plus S. cerevisiae (grey branches). The bar graphs show the respective genome sizes and the number of proteins used (colored) and folded for our structural comparison. b) Schematic pipeline of our structural similarity approach, from protein structure prediction with ColabFold (v1.5.2) to structural matching using Foldseek (v5-53465f0), followed by a manual curation step with ANNOTEX that includes a comparison of sequence and structure-based hits to achieve a high-quality functional annotation