Fig. 3
From: Functional annotation of a divergent genome using sequence and structure-based similarity
Complementation of structure and sequence-based functional annotation enriches the total number of matches and improves the annotation of microsporidia-specific genes. (a) To assess the annotation efficiency of our combined structure and sequence-based similarity approach, we counted the amount of identical (green), non-identical (dark grey), not identified (light grey) and experimentally determined (black) functional gene predictions between ANNOTEX and ProtNLM. Additionally, we display the relative number of potential miss-annotations (dark grey with black dashed line) predicted by ProtNLM and the percentage of ProtNLM gene function predictions with a model score above 0.2 (dark green dashed line) that we transferred to genes which our approach suggested to be uncharacterized or hypothetical. (b) Employing our approach, we functionally annotated 12% (dark green) and characterized the domain of 7% (green) of the 381 uncharacterized E. cuniculi proteins. RBLL-1, ricin B lectin-like 1