Fig. 4

Model validation. A Comparison of growth predictions using iSH1474 (Simulation) and experimental growths on 190 carbon sources contained in the phenotype microarray and 13 organic compounds (Experiment). The number in the parenthesis denotes the number of growth predictions using iJN1462. B Comparison of MEMOTE reports of iSH1474 (S12) and iJN1462 (KT2440). C Comparison of flux distribution in the central carbon metabolism of S12 from in vivo measurements and in silico predictions. Reported experimental flux values (on the x-axis) are compared with the predicted fluxes (y-axis) using iSH1474 (S12) and iJN1462 (KT2440). The flux values are normalized to the experimental glucose uptake rate. D Dynamic flux balance analysis using iSH1474 for time profiles of biomass and glucose concentration in aerobic batch growth of S12 on glucose as the sole carbon source. Black and white circles denote experimental data of biomass and glucose concentration in the culture medium [39], respectively. Solid and dashed red lines represent the model predictions for biomass and glucose concentration, respectively