Fig. 5

Model predictions of growth capability of S12 growing aerobically on heptanoate, octanol, styrene, and glucose. A The simulated catabolic routes. Metabolites are in the box, and metabolic reactions are in italic bold. Arrows denote directions of the predicted metabolic fluxes, and dashed arrows indicate the multi-step reaction. Metabolites colored above the arrows indicate cofactors of ADP, ATP, NAD(P), and NAD(P)H. The metabolites (acetyl-CoA [accoa], glyceraldehyde 3-phosphate [g3p], succinyl-CoA [succoa], and pyruvate [pyr]) duplicated on the map are labeled with an asterisk. Each catabolic route is indicated by a different color. B Predicted growth rate, ATP production, and NAD(P)H regeneration according to the carbon source utilized. “Reactions for ATP production” and “Reactions for NAD(P)H regeneration” are reactions responsible for > 90% of total ATP production and total NAD(P)H regeneration, respectively, in descending order. For flux balance analysis, the upper limits of oxygen and each carbon source were set to 18.5 and 10 mmol/gDCW/h, respectively. Abbreviations are given in Table S2