Fig. 5

EDNRB haplotypes in pigs with diverse pigmentation patterns and candidate causative mutation for the two-end‐black coat color phenotype in pigs. A: Haplotype block analysis at the most significant signal from 50.086 to 50.089 Mb on SSC11. B: Network of EDNRB haplotypes with frequencies greater than two. The haplotypes were defined from 25 SNPs within the EDNRB region of 1.2 kb. C: Frequencies of the 1.2‐kb EDNRB haplotype in TEB and non-TEB color pigs. D: Genome browser view of the differential ATAC-seq peak in the EDNRB gene. The blue box indicates that significantly selected SNPS are located within the ATAC-seq peak region of the skin, but not in the ATAC-seq of muscle tissue. E: Relative luciferase activity comparison between the 1.2‐kb EDNRB haplotype of TEB and non-TEB color pigs in the porcine kidney cell line, PK15 cells. The 1.2‐kb EDNRB haplotype regions of Jinhua pigs and Xidu black pigs were cloned into pGL3-Promoter vectors. pGL3-Promoter was used as controls. The bars display mean ± SD (N = 3 technical replicates). F: A working model showing how the 1.2‐kb EDNRB haplotype affects the TEB coat color in pigs