Insights into the genetic variability and evolutionary dynamics of tomato spotted wilt orthotospovirus in China

BMC Genomics

Table 6 Primers designed for full genome sequence analysis of TSWV

Serial No.	Primer name	Sequence (5′-3′)	Amplicon size (bp)	Tm (℃)^a
1	TSWV-L-1 F	AGAGCAATCAGGTAACAAC	1257	51
1	TSWV-L-1R	TTTTGGATTAAGTGGTCTTTC	1257	51
2	TSWV-L-2 F	GAAGATATTGAAAGAATAATTGATTC	1101	50
2	TSWV-L-2R	ATTCAGGATTTTTAGCATAATTATTG	1101	50
3	TSWV-L-3 F	CAAGTATAGACATGTCTTCTCTGACTC	1286	55
3	TSWV-L-3R	CCTAGATACTAATCAGATAATGCTTTC	1286	55
4	TSWV-L-4 F	TGAAGACATATACCCTAAGAAAGC	976	52
4	TSWV-L-4R	CAGATAGGAAAGCCAATCTTG	976	52
5	TSWV-L-5 F	CATCGGAAGCCATATCTATAAG	1158	54
5	TSWV-L-5R	GCTCTCTGAATCTCATCTGTAGATA	1158	54
6	TSWV-L-6 F	CTCCTATAGAGCCGTTGTCTATATTAG	1161	55
6	TSWV-L-6R	ATCATATAATTGCATGCTTTCACC	1161	55
7	TSWV-L-7 F	TTGGAACAGGTTTAATCATGG	1276	52
7	TSWV-L-7R	TTCTCTGATATCATCATCTACAATCTC	1276	52
8	TSWV-L-8 F	GAATGACCACAGACAACAAAATG	1131	52
8	TSWV-L-8R	CAATCAAGAACTTTATCAACTCACTTAG	1131	52
9	TSWV-L-9 F	GTGTTGAGGCTAGATGAGGAAG	916	55
9	TSWV-L-9R	AGAGCAATCAGGTACAACTAAAAC	916	55
10	TSWV-M-1 F	AGAGCAATCAGTGCATCAGA	955	55
10	TSWV-M-1R	CTTCTTCTTCAACTGATCTCTCAAG	955	55
11	TSWV-M-2 F	GCAAGCTGATAATTCCTAAAGG	1351	55
11	TSWV-M-2R	AAGGAGATGACATGTCTTGGG	1351	55
12	TSWV-M-3 F	CCGCATAGAAGACAGCC	1276	55
12	TSWV-M-3R	GTTATAGAAGGTCCTAATGATTGCA	1276	55
13	TSWV-M-4 F	GTTAACCCTAAAGAGCTTCCTG	979	52
13	TSWV-M-4R	GAGAAGATCATGGGTTATTTGAT	979	52
14	TSWV-M-5 F	CTTATCCAAGAAAAATTGATGC	1051	50
	TSWV-M-5R	AGAGCAATCAGTGCAAACAAAA	1051	50
15	TSWV-S-1 F	AGAGCAATTGTGTCATAATTTTATTC	1258	52
15	TSWV-S-1R	TTGCAGATATCTTCACTGTAATTTAAG	1258	52
16	TSWV-S-2 F	CTCTGCTTGAAACTCACACATC	1097	52
16	TSWV-S-2R	AAATGAATGAAGATCAGGTGAAG	1097	52
17	TSWV-S-3 F	YCCATAGCAATACTTCCTTTAGC	848	50
17	TSWV-S-3R	AGAGCAATTGTGTCAATTTTATTC	848	50

ISSN: 1471-2164