Fig. 2

Expression Specificity Score (ESS) quantifies cell-type specific expression. A Schematic of the ESS scale and its interpretation in the context of pancreatic expression levels. Cartoons of cells represent distinct pancreatic cell-types, grey shading indicates expression levels of glucagon (GCG) or actin (ACTB) in these cell-types. UMAP plots of α-cell specific expression of glucagon gene (GCG) and the housekeeping gene actin (ACTB) are shown. The latter UMAP shows moderate expression of this gene in all cell types. B Heatmap of ESS values for representative known markers for each cell type, along with housekeeping genes, to demonstrate the concordance of ESS with expected values in known targets. C Density plot of ESS values for all genes in each cell type. D Bar chart of specificity scores for known markers using the ESS and Gini metrics. E Scatter plot comparing ESS in scRNA-Seq to ESS in bulk sorted beta cells, calculated using α-, β-, acinar, and duct cell data. Results for the top expressed 500 genes in beta cells are shown