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Fig. 4 | BMC Genomics

Fig. 4

From: In-depth transcriptomic analysis of Anopheles gambiae hemocytes uncovers novel genes and the oenocytoid developmental lineage

Fig. 4

Lineage analysis to determine hemocyte differentiation. (a) Lineage of hemocyte clusters defined using the genome (P4.14 version) annotated transcripts as a reference by unsupervised analysis using the TSCAN software. (b) Pseudotime plot of the same clusters analyzed with Monocle3, using PHem3 as root. (c) Lineage map of hemocyte clusters defined with the hemocyte transcriptome as a reference by unsupervised analysis using the TSCAN software. (d) Pseudotime plot of the same clusters with Monocle3, using PHem3 as root. (e) Schematic summary of the lineage of hemocyte subpopulations based on the differentiation pathways determined by Moncole3 pseudotime analysis based on hemocyte transcriptome annotation as a reference

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