Table 2 Primers for identification of wzy deletion
From: Genome-wide association study reveals serovar-associated genetic loci in Riemerella anatipestifer
Name of pimer | Targeted gene;Description | Product Length(bp) | Â | Sequence | Source or reference |
|---|---|---|---|---|---|
wzy-Left | G148_RS04360; Amplification of the wzy upstream fragment | 609 | F | AAGAACATTACCCATATCCTATCGTTTCGACGGTA | This study |
R | TTCTGTCCTGGCTGGTTTTACGAATATTTGTAAGATA | This study | |||
wzy-Right | G148_RS04370; Amplification of the wzy downstream fragment | 602 | F | CCAAGGTAGTCGGCAAATAATTTTATGAAAAAAGTAC | This study |
R | TACATGAGAAACCACAAAAGCCTCTTTGGGAATA | This study | |||
wzy | RA-CH-2 (G148_RS04365); Amplification of the wzy | 886 | F | TCCAATGGGTTTACTTTCTTGTAACTTTGTCT | This study |
R | CGTAATGGTTGGTTGAGATTCATTGGAG | This study | |||
LSR | G148_RS04360 + spec; Identification of transconjugants | 1199 | F | AGGTAGATAGGGCAAGTATGGCTTTTTCG | This study |
R | ACCGTAACCAGCAAATCAATATCACTGTG | This study | |||
16S rRNA_RA | 16S rRNA; Identification of species | 960 | F | CTTCGGATACTTGAGAGCG | [28] |
R | GCAGCACCTTGAAAATTGT | [28] | |||
Spec | spec; Amplification of the spec | 1145 | F | TCTTACAAATATTCGTAAAACCAGCCAGGACAGAAAT | This study |
R | ACTTTTTTCATAAAATTATTTGCCGACTACCTTGGTG | This study |