Table 2 Primer sequences used in the species-specific PCR assay
Gene | Primers | Sequences (5’-3’) | Length (bp) | Production (bp) | Annealing (°C) |
|---|---|---|---|---|---|
DNA barcoding | LCO1490 | GGTCAACAAATCATAAAGATATTGG | 25 | / | 43 |
HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA | 26 | |||
COI | HLCOIF | CTTCTTTAACCTTTCTCT | 18 | 474 | 55 |
HLCOIR | TTTTTCCTCTCTCTTGTC | 18 | |||
ND2 | HLND2F | TTCTGGCTCCCTGAAGTA | 18 | 434 | 55 |
HLND2R | CCTAAAAATGGGGGTAAA | 18 | |||
ND4 | HLND4F | TTTTAGGATGAGGTAGACA | 19 | 346 | 56 |
HLND4R | ATACCAAAATCAATAAACAA | 20 | |||
ND5 | HLND5F | TGCCCTATCTAACCGTGT | 18 | 452 | 56 |
HLND5R | ATTCTGCCCCACCTACAC | 18 |