Fig. 1

SC-islets share endocrine cell types with fetal and adult islets, see also Tables S1 & S2. a Schematic of 19 human adult islets, male and female, age 19-56; 9 human fetal pancreases, male and female, 110-122 dpc; SC-islets derived from 4 unique protocols; and SC-islets derived from 2 unique protocols and transplanted into the kidney capsules of mice for 1-6 months. Each individual dataset is plotted onto a UMAP which indicates scaled expression of CHGA after quality control filtering. b UMAP of all endocrine cells integrated from each published dataset with 10 unique cell types identified. Polyhormonal (Poly), Endocrine Progenitor (Prog), Proliferating Endocrine (Prolif), Enterochromaffin-like (EC), Neuroendocrine (NE). c Proportion of identified cell-types from SC, SC-TXP, adult, and fetal islet sources. d Integrated endocrine UMAP split between SC, SC-TXP, adult, and fetal sources. e Feature plots indicating scaled expression level of various islet cell hormones. f Heatmap indicating top differentially expressed genes for each endocrine cell population. g Pairwise analysis indicating differentially expressed genes (log2FC > 0.3) between β-cells and all other endocrine cells shared between SC, SC-TXP, fetal, and adult islets. DEGs enriched in β-cells from all four sources make up core β-cell identity