Fig. 1

Binding profiles of Pnt ChIP-seq replicates and snATAC-seq show clear enrichment at known targets of Pnt. (A) Schematic of Epidermal growth factor receptor (Egfr) pathway and cell type differentiation in larval eye discs. Induction of Egfr by Spitz (Spi) triggers the activation of Ras, which in turn activates Extracellular signal regulated kinase (Erk). Erk translocates to the nucleus and activates Pointed (Pnt), which mediates the transcriptional output of Egfr pathway. Except R8, all cell types undergo differentiation upon Egfr activation. (B) Schematic of tangential sections of an adult ommatidium showing different cell types. 1° = primary pigment cells; 2° = secondary pigment cells; 3° = tertiary pigment cells; and B = bristle cells. (C) Late larval scRNA-seq plot showing clusters corresponding to all major cell types present in the physical eye disc. (D) Plot showing the expression pattern of hh from single cell RNA sequencing (scRNA-seq) data. The intensity of blue is proportional to log-normalized expression levels. (E) The hedgehog (hh) locus with ChIP-seq peaks overlapping an enhancer reported to be bound by Pnt (black rectangular box). FLAG1, FLAG2, FLAG3, GFP1 and GFP2 are Pnt ChIP-seq biological replicates. (F) snATAC-seq genomic track showing the hh locus with peaks that overlap the known enhancer and peak shown in (E). Predicted Ets binding sites are shown as red triangles