Fig. 3

SQANTI3 classification for detected isoforms between sample prep methods. The samples include Human Brain Reference RNA mixed with Spike-In RNA Variants “SIRV” prepared with the PacBio Full-Length Isoform Concatemer Sequencing workflow “HBR_SIRV_Con” or prepared with a non-concatemer Iso-Seq workflow “HBR_SIRV_Non”. The pie graph illustrates the number of detected unique genes for the HBR_SIRV_Non and HBR_SIRV_Con samples, in which unique genes are defined as reference-based annotated genes and those genes curated from sample specific novel isoforms. The bar graph illustrates categorization of identified isoforms using the SQANTI3 classifier. SQANTI3 classes include Full-Splice Match (FSM; isoform aligns completely to reference), Incomplete-Splice Match (ISM; isoform partially aligns to a known reference transcript), Novel_in_Catalog (NIC; isoform containing novel combinations of known donor and acceptor splice sites), Novel_not_in_Catalog (NNC; isoforms containing at least one unannotated splice site), Genic Genomic (isoform containing partial exon and intron/intergenic overlap), Antisense (isoform containing overlap with complementary strand of an annotated transcript), Fusion (isoforms spanning two annotated loci), Intergenic (isoform aligns completely outside of annotated gene), and Genic Intronic (isoform aligns entirely within an annotated intron)