Table 2 Primers used in this study
Primer Name | Sequences (5’→3 ‘) | Length | Gene | Gene ID | Purpose | |
|---|---|---|---|---|---|---|
Rdhsp1-F | GACGGAATCGACTTCTACACC | 129 | rdhsp1 | B3-1_1-10k_transcript/15,170 | RT-qPCR | |
Rdhsp1-R | GATTGACCCTTTGTCCATTTT | |||||
Rdhsp2-F | CAACAACCTCTTGGGTACTTTCG | 120 | rdhsp2 | B3-1_1-10k_transcript/15,497 | RT-qPCR | |
Rdhsp2-R | TCCTTCGCAGATACGTTCAGG | |||||
Rdfa1-F | GTACTCATTCACGCTGGTAGTGG | 104 | rdfa1 | B3-1_1-10k_transcript/10,065 | RT-qPCR | |
Rdfa1-R | CTCTTTGCTTGACTGCCGACT | |||||
Rdfa2-F | CGGTACTCATTCACGCTGGTA | 106 | rdfa2 | B3-1_1-10k_transcript/1385 | RT-qPCR | |
Rdfa2-R | CTCTTTGCTTGACTGCCGACT | |||||
Rdpla1-F | CTTCTGCTGGTGCCTATT | 97 | rdpla1 | B3-1_1-10k_transcript/9998 | RT-qPCR | |
Rdpla1-R | CGAAAGTCGTATGGTGCT | |||||
Rdpla2-F | ATACCAGGATTCGTTTGG | 96 | rdpla2 | B3-1_1-10k_transcript/22,992 | RT-qPCR | |
Rdpla2-R | TTCTTGATGGATAGGGACT | |||||
Rdtpst1-F | GTGGCACAAGATAACAACAGA | 145 | rdtpst1 | B3-1_1-10k_transcript/20,862 | RT-qPCR | |
Rdtpst1-R | GAGAAGCGGCAGGTAGAA | |||||
Rdtps1-F | TAAAGGTGCCCTGCTGAC | 100 | rdtps1 | B3-1_1-10k_transcript/7670 | RT-qPCR | |
Rdtps1-R | GAACCCTGCTTCTTCAATC | |||||
Rdvg1-F | CCCATAATGAACAGTCCACAA | 140 | rdvg1 | B3-1_1-10k_transcript/14,375 | RT-qPCR | |
Rdvg1-R | TGATACAATCTTCCGTAATCCA | |||||
Rdvg2-F | TCGTCAGCGACAGCCAGGTC | 102 | rdvg-2 | B3-1_1-10k_transcript/26,394 | RT-qPCR | |
Rdvg2-R | TCTTGTGCGGAGCGCAGTTC | |||||
Rdrps3-F | TGGGACCCTAATGGCAAGA | 137 | rdrps3 | B3-1_1-10k_transcript/26,326 | RT-qPCR | |
Rdrps3-R | GCAACAGGTGGTATGTCTGATTT | |||||
dsTPS-F | GGATCCTAATACGACTCACTATAGGTCTACAGGGATGCTGCAGTG | 486 | rdtps1 | B3-1_1-10k_transcript/7670 | RNAi | |
dsTPS-R | GGATCCTAATACGACTCACTATAGGCCAAATCAGGATGAGGTGCT | |||||
dsGFP-F | GGATCCTAATACGACTCACTATAGGGATTAAGTTCAGCGTGTCCG | 428 | gfp | (Qiu et al., 2015) | RNAi | |
dsGFP-R | GGATCCTAATACGACTCACTATAGGCTAGTGATTCACCTTGATGC | |||||
dsHSP2-F | GGATCCTAATACGACTCACTATAGGGGGCACGATTCGAAGAATTA | 445 | rdhsp2 | B3-1_1-10k_transcript/15,497 | RNAi | |
dsHSP2-R | GGATCCTAATACGACTCACTATAGGCTCTCACCCTCGAAGACCTG | |||||
dsFAS2-F | GGATCCTAATACGACTCACTATAGGTATTGGAAATTCGCGTGACA | 465 | rdfa2 | B3-1_1-10k_transcript/1385 | RNAi | |
dsFAS2-R | GGATCCTAATACGACTCACTATAGGGCAGTGACCGTTTTTGGATT | |||||
dsPLA1-F | GGATCCTAATACGACTCACTATAGGTACCTGGTGATGGTGGTTCA | 493 | rdpla1 | B3-1_1-10k_transcript/9998 | RNAi | |
dsPLA1-R | GGATCCTAATACGACTCACTATAGGTACAAAGACATTGGGCCTCC | |||||
dsTPST-R | GGATCCTAATACGACTCACTATAGGGCCGCTTCTCTTGTTTTGAC | 441 | rdtpst1 | B3-1_1-10k_transcript/20,862 | RNAi | |
dsTPST-F | GGATCCTAATACGACTCACTATAGGCATCCCGATACCAGAGAGGA | |||||
dsVG-F | GGATCCTAATACGACTCACTATAGGATATCTACCCACTGCCACGC | 425 | rdvg1 | B3-1_1-10k_transcript/14,375 | RNAi | |
dsVG-R | GGATCCTAATACGACTCACTATAGGCTTCTGCACGGTGAAACAGA | |||||
dsP450-F | GGATCCTAATACGACTCACTATAGGTTGATCCTTTCATGGGTGGT | 411 | rdP450 | B3-1_1-10k_transcript/18,550 | RNAi | |
dsP450-R | GGATCCTAATACGACTCACTATAGGTGCAATCCTTTGGGAAGAAC | |||||