Fig. 2
From: The impact of selective HDAC inhibitors on the transcriptome of early mouse embryos
Outline of the effects of HDACi on early embryos. (a) Heatmap of the correlation between the HDAC inhibitors (HDACi) treatment and the control groups at three developmental stages. The mean of Pearson’s correlation coefficients of replicate samples within the treatment is shown. The red crosses indicate embryos that failed to develop to the corresponding stage after HDACi treatment. Results of the principal component analysis of combined data from other normal pre-implantation embryos (b) and of data from the HDACi treatment and control groups alone (c). The colors indicate the different developmental stages, and the shapes indicate the different treatment conditions. (d) The bar graph shows the genes with the highest loadings in the PC3 direction in (c), where red indicates a positive correlation between gene expression and the HDACi effects, and blue indicates a negative correlation. (e) Bar graph showing the 10 gene ontology (GO) terms with the highest absolute NES values in the enrichment analysis, using the loading order of genes in the PC3 direction in (c). NES: normalized enrichment score; CoreGene: genes in the ranked gene list before (for positive ES) or after (for negative ES) the peak in the running enrichment score