Fig. 4

Profile of the HDACi effects on 2-cell stage embryos. (a) Volcano plot of DEGs, as in Fig. 3a. (b) Heatmap of DEGs prepared using the hierarchical clustering method, to divide the differential genes into five clusters. (c) The network diagram represents the enriched biological processes within each group of DEGs and their relationship. Each point represents a GO term that has been enriched, with its size indicating the negative logarithm of the P-value obtained from the enrichment analysis. Different colors represent different GO-term clusters, with each cluster being annotated with a label, and clusters with the same label being represented by the same color in the networks of the different groups. The width of the lines connecting the points represents the strength of the relationships between the GO terms, with brown lines connecting a pair of GO terms in which one is a subset of genes contained in the other. The layout of the network indicates that GO terms with a higher degree of correlation tended to cluster together