Fig. 2

Volcano plot summarizing the differentially expressed sequences in female vs. male antennae of D. longicaudata. Transcript abundance is represented as Log₁₀ (TPM + 1). The x-axis and y-axis represent Log₂ fold-change (Log₂FC) differences between the contrasted groups and statistical significance as the negative Log₁₀ of adjusted P-value (s-value), respectively. A total of 13,342 genes were included in the analysis, yielding 237 and 151 transcripts, up- and downregulated in the female antenna vs. male antenna. Transcripts upregulated (Log₂FC > 1 and s-value < 0.05) are indicated with red dots, those downregulated (Log₂FC < 1 and s-value < 0.05) are highlighted in blue, and non-significant transcripts are shown as gray dots (n = 5 biological replicates per group). In addition, sensory-related genes that were differentially expressed between groups are highlighted as follows: odorant receptors (OR) in green, odorant-binding proteins (OBP) in purple, chemosensory proteins (CSP) in yellow, and transient receptor potential channels (TRP) in orange. Log₂FC < 1 and s-value < 0.05 for all transcripts analyzed are detailed in Table S4: Sheets A and B