Fig. 10

Analysis of the subcellular localization of ZmPUB19 and ZmPUB59. ZmPUB19-GFP and ZmPUB59-GFP colocalized with NLS-mCherry. Paired constructs were infected into Nicotiana leaves. GFP and mCherry fluorescence signals were monitored 40–48 h after infection by confocal microscopy. For each construct, 10–20 cells were analyzed, and a similar nuclear localization pattern was observed. ZmPUB19-GFP and ZmPUB59-GFP had strong fluorescence expression signals in the nucleus and coincided with the NLS-mcherry expression signal in the nucleus, confirming that ZmPUB19 and ZmPUB59 were mainly localized in the nucleus. Scale bar, 20 μm