Fig. 4

Analysis of the conserved motifs and gene structure of the U-box family in maize. A The phylogenetics, distribution of conserved motifs. The Neighbor-Joining (NJ) phylogenetic tree of 85 maize PUB genes, which were constructed using the MEGA7 software (Neighbor-Joining, NJ) with the bootstrap parameter set to 1000. The subfamily information is represented by G1-G4. Based on MEME online analysis, the 20 conserved motifs of the U-box proteins in maize were designated motifs 1 ~ 20 and are represented by different colors. B Distribution of gene structure. The exon/intron structure of ZmPUB genes was determined by comparing CDS with genomic sequences. Green and yellow rectangles represents untranslated region (UTR) and coding DNA sequence (CDS) features, respectively, the horizontal line represents gene regions, and gray lines represents introns