Fig. 7
From: Single-cell RNA-seq mapping of chicken peripheral blood leukocytes
Identification of chicken leukocytes in the indicated populations by phenotypical identification by immunofluorescence labelling and flow cytometric analysis (FACS; blue bars) and by single-cell mRNA expression (mRNA; pink bars). For phenotypical identification, cells were identified as cells in the “lymphocyte gate” with cell surface expression of Bu-1, CD4, CD8α, CD8β, TCRγ/δ and TCRα/β (combination of TCRα/Vβ1 and TCRα/Vβ2), respectively. Monocytes were identified by FSC/SSC characteristics and cell surface expression of MMR1L4 (MRC1L-B) and heterophils were identified by FSC/SSC characteristics and cell surface expression of CD45. Data was expressed as proportions of populations out of live events for each individual hen. For gating strategies see Additional file 6. For mRNA identification, cells with log2 fold change ≥ 0.25 expression of LOC396098 (Bu-1), CD4, CD8A (CD8α), CD8BP (CD8β) and MMR1L4 were considered positive. For TCRγ/δ, cells in clusters 1, 0, 23, 25 and 29 were considered positive, for TCRα/β cells in clusters 3, 8, 11, 12, 13, 14, 15, 16, 24, 30 and 7 were considered positive and for heterophils cells in monocyte subcluster 2 were considered positive. Data was expressed as proportions of positive cells out of the total number of cells after filtering for each individual hen. Results shown are means ± 95% confidence intervals, n = 4