Fig. 6
PCR confirmation of transgenic wheat harboring the TtMYB1 gene. PCR detection of the TtMYB1 gene in the genomic DNA of putative transgenic T0 wheat leaves. The fragment of the amplified TtMYB1 gene is indicated by a yellow arrow. A white arrow shows the band of the amplified housekeeping 18 S gene. M, 2,000 bp DNA marker; lanes 1, 5, 8, 10, and 12, transgenic plants; lanes 2, 3, 4, 6, 7, 9, and 11, non-transformed plants; P, positive control (TtMYB1 recombinant plasmid); N, negative control (wild-type DNA)