Table 2 The statistics of RNASeq mapping. (Clean Reads: the total number of sequences used for alignment. Total_Mapped: compare the total number of sequences in the reference genome, the percentage represents the ratio of total mapped to clean reads. Multiple_Mapped: compare the total number of sequences to multiple locations, the percentage represents multiple mapped/total mapped. Uniquely_Mapped: the total number of sequences aligned to only one position, percentages represents uniquely mapped/total mapped. The values 25, 30, and 35 correspond to temperatures of 25 ℃, 30 ℃, and 35 ℃ respectively, CK: 75%-80% FC, Z: 30%-35% FC)
Sample | Clean_Reads | Total_Mapped | Multiple_Mapped | Uniquely_Mapped |
|---|---|---|---|---|
T25CK1 | 38,348,600 | 29,976,384 (78.17%) | 3,895,388 (12.99%) | 26,080,996 (87.01%) |
T25CK2 | 37,924,222 | 29,726,811 (78.38%) | 3,839,436 (12.92%) | 25,887,375 (87.08%) |
T25CK3 | 36,433,556 | 28,328,324 (77.75%) | 3,675,909 (12.98%) | 24,652,415 (87.02%) |
T25Z1 | 34,832,706 | 27,470,506 (78.86%) | 3,497,848 (12.73%) | 23,972,658 (87.27%) |
T25Z2 | 43,923,726 | 34,416,797 (78.36%) | 4,447,244 (12.92%) | 29,969,553 (87.08%) |
T25Z3 | 39,865,744 | 31,362,974 (78.67%) | 3,944,800 (12.58%) | 27,418,174 (87.42%) |
T30CK1 | 36,920,674 | 28,845,654 (78.13%) | 3,748,375 (12.99%) | 25,097,279 (87.01%) |
T30CK2 | 37,041,144 | 28,928,651 (78.10%) | 3,749,472 (12.96%) | 25,179,179 (87.04%) |
T30CK3 | 38,429,136 | 29,851,865 (77.68%) | 3,935,189 (13.18%) | 25,916,676 (86.82%) |
T30Z1 | 42,492,918 | 32,677,002 (76.90%) | 4,098,846 (12.54%) | 28,578,156 (87.46%) |
T30Z2 | 42,609,752 | 32,250,497 (75.69%) | 4,166,230 (12.92%) | 28,084,267 (87.08%) |
T30Z3 | 42,799,492 | 32,670,829 (76.33%) | 4,230,763 (12.95%) | 28,440,066 (87.05%) |
T35CK1 | 37,510,860 | 28,752,738 (76.65%) | 3,736,957 (13.00%) | 25,015,781 (87.00%) |
T35CK2 | 42,351,116 | 32,342,688 (76.37%) | 4,174,159 (12.91%) | 28,168,529 (87.09%) |
T35CK3 | 41,485,354 | 31,715,253 (76.45%) | 4,101,931 (12.93%) | 27,613,322 (87.07%) |
T35Z1 | 40,300,800 | 31,327,335 (77.73%) | 4,374,634 (13.96%) | 26,952,701 (86.04%) |
T35Z2 | 38,982,238 | 30,043,717 (77.07%) | 4,027,367 (13.41%) | 26,016,350 (86.59%) |
T35Z3 | 40,852,800 | 31,547,932 (77.22%) | 4,704,806 (14.91%) | 26,843,126 (85.09%) |