Fig. 6

(A) Molecular signatures of Eomes⁺ germ cells from mice after busulfan treatment for 8 days. Cell cycle analysis of Eomes⁺ germ cells from Eomes-TMs was performed using Seurat, reputation = t-SNE. The G1, S and G2/M phases are indicated by the corresponding colors. (B) t-SNE plot of integrated Eomes + spermatogonia from Eomes-Ctr and Eomes-TM. Each dot represents a single cell, and cell clusters are distinguished by color. (C) Volcano plot of genes differentially expressed between the Eomes-TM and Eomes-Ctr groups (adjusted p value < 0.05; fold change > 2). Red indicates upregulated genes, and blue indicates downregulated genes. (D) Enriched KEGG terms for DEGs in Eomes + spermatogonia from the Eomes-TM cohort. (E) Pseudotime trajectory analysis of integrated Eomes + spermatogonial subsets defined in clusters. Cluster 4 was the developmental origin subpopulation. Cells are colored according to the pseudotime score, with dark colors representing immature stages and light colors representing mature stages. (F) Heatmap of 3 distinct states of pseudotime-dependent genes and enriched KEGG terms for each temporal state. Blue indicates low expression, and red indicates high expression