Fig. 2

Distribution of 6mA in A. veronii genome. A Percentage of 6mA sites across different types of genes. Each color corresponded to a specific gene type. B Percentage of genes with and without 6mA. C Frequency of 6mA sites at each relative position within protein-coding genes. D Number of stop codons with and without 6mA. E Frequency of 6mA sites at each relative position within non-coding RNA genes. F Frequency of 6mA sites at each relative position within intergenic regions. In (C), (E), and (F), if a gene/intergenic region had N bases, the relative position of the base at the i-th position was represented as i/N, ranging between 0 and 1. G Comparison of 6mA frequencies in different relative segments within intergenic regions. The relative positions (0–1) within the intergenic region were divided into three segments, 0.0–0.1, 0.1–0.9 and 0.9–1.0. The bases in the 0.0–0.1 and 0.9–1.0 segments were in close proximity to the gene body. P-values were calculated by Wilcoxon test. No significant difference in 6mA frequency was observed between the 0.0–0.1 and 0.1–0.9 segments (P value > 0.01). H Frequency of 6mA sites at each position in the upstream 50 bp of transcription start site (TSS)