Fig. 2

In vitro validation of the effect of liraglutide on C2C12 under high glucose condition. A: MTT was used to detect cell viability in blank control, mannitol treated, high glucose, liraglutide (100 nmol/L) + high glucose, liraglutide (200 nmol/L) + high glucose and liraglutide (500 nmol/L) + high glucose groups; B: Protein expression bands of AMPKα, MAFbx and MuRF1 in blank control, mannitol treated, high glucose and liraglutide (200 nmol/L) + high glucose groups. The blots were cropped and the full-length blots/gels are presented in Supplementary material-Original blot. C: Statistical histogram of AMPKα protein expression in blank control, mannitol treated, high glucose and liraglutide (200 nmol/L) + high glucose groups; D: Statistical histogram of MAFbx protein expression in blank control, mannitol treated, high glucose and liraglutide (200 nmol/L) + high glucose groups; E: Statistical histogram of MuRF1 protein expression in blank control, mannitol treated, high glucose and liraglutide (200 nmol/L) + high glucose groups; F: The content of 3-MH in the cell supernatant of blank control, mannitol treated, high glucose and liraglutide (200 nmol/L) + high glucose groups was detected by ELISA. *, P < 0.05; **, P < 0.01; ***, P < 0.001