Fig. 1

Graphical overview of the study design. A Biological material used in the study; B Sequencing analyses performed during the study; C Technical-replicates-based comparisons using F1 score calculations, including the pairwise-triplicate-based, TDS-based, and blood–saliva-based comparison; D Cross-protocol-based comparisons of concordance rates; E Sequencing-metrics-based comparisons; F Contamination rate evaluation