Table 1 Summary of abiotic and biotic exposure treatments used for fragments of Orbicella faveolata to generate as full as possible transcriptional snapshot for annotated transcriptome generation. Ten samples were successfully extracted, clean and concentrated, equally pooled and sent for ISO-seq library preparation and sequencing on one flow cell of a Pacbio Sequel II.

ofav_rna_1

cold stress

Fragment placed at 20ºC for 2 h

2.01

195

ofav_rna_2

fed

Fragment fed with Reef Roids (PolypLab) for 1 h

2.09

87.6

ofav_rna_3

heat stress

Fragment placed at 33ºC for 2 h

1.93

55.3

ofav_rna_4

fighting

Fragment with mesenterial filament extension when next to a different genot of O. fav

1.92

186

ofav_rna_5

night

Fragment sampled at 11pm local time

1.89

121

ofav_rna_6

day

Fragment sampled at 11am local time

2.13

117

ofav_rna_7

sperm

Preserved sperm used for HMW DNA

2.16

35

ofav_rna_8

hypersalinity

Fragment placed in 45ppt for 2 h

1.96

59.6

ofav_rna_9

disease

Fragment with visible SCTLD lesion

2

189

ofav_rna_10

hyposalinity

Fragment placed in 20ppt for 2 h

2.01

63.6