Fig. 7

Verification of RNA-seq by qRT-PCR and the correlation analysis. A Expression patterns of 5 selected genes measured using the 2^−ΔΔCT method. The histograms in cyan represented RT-PCR results with Error bars showing the means ± SEM. The orange lines represented the average values of FPKM for each sample. RC, RT, SC, and ST are samples of one high resistance (R, Harmonie) and one highly susceptibility (S, Carefree Wonder) rose cultivar with (T) or without (C) aphid infestation. B Correlation analysis for 5 selected genes between RNA-Seq and qRT-PCR