Fig. 1

Different expression analysis in genes of New Zealand white rabbit ovarian granulosa cells treated with DMSO and MLN4924. A PCA score plot of transcriptomes. PC1 and PC2 coordinates represent the first and second principal components, respectively, with percentages indicating their contributions to sample variance. The control group samples are represented in blue, while the MLN4924 treatment group samples are represented in red. B The number of up-and down-regulated differentially expressed genes (DEGs). C Volcano plot for DEGs. The x-axis represents the logarithmic fold change between the two groups, and the y-axis represents the negative log10 value of the FDR for the group difference. Red points indicate up-regulated expression level in the MLN group compared to the DMSO group, blue points indicate down-regulated expression and gray points indicate no difference (criterion: log₂FC ≥ 1.2, P < 0.05). D The heatmap shows the relative expression of DEGs. In the diagram, each column represents a sample, and each row represents a gene. Gene expression in different samples is depicted in varying colors. The redder the color, the higher the expression level, whereas the bluer the color, the lower the expression level. E Pearson correlation between samples. The left and upper sides show the clustering situation of the samples, while the right and lower sides of the graph show the sample names. The numbers indicate the correlation coefficient between the samples