Fig. 2

Functional enrichment analysis of DEGs in GCs of New Zealand white rabbits treated with DMSO and MLN4924. A Significantly up-regulated genes were examined using GO pathway enrichment (P < 0.05) histogram statistical analysis. B Significantly down-regulated genes were examined using GO pathway enrichment (P < 0.05) histogram statistical analysis. The x-axis represents the GO Term, while the y-axis represents the -log10 (P-value) enrichment of the GO Term. The orange color indicates a biological process, and the blue color indicates cellular component. C Scatter plot statistical analysis of significantly up-regulated genes using KEGG pathway enrichment (P < 0.05). D Significantly down-regulated genes were examined by using KEGG pathway enrichment (P < 0.05) scatter plot statistical analysis. The size of the point represents the number of enriched genes, and the color intensity indicates the significance level (the redder the color, the smaller the P value). E PPI analysis of DEGs using Cytoscape software. Orange circles represent up-regulated genes, and green circles represent down-regulated genes, arranged by betweenness centrality. The greater the centrality of the number, the larger the circle. F Interaction networks of differentially expressed proteins in major pathways significantly altered by MLN4924 treatment. Orange nodes indicate upregulated proteins and green nodes indicate downregulated proteins