Fig. 3

Verification of differentially expressed genes. GCs were seeded in 12-well plates and treated with DMSO or MLN4924 (1 µM) for 24 h. Cells were harvested, and gene expression was analyzed using RT-qPCR. β-actin served as a standardized internal control. N = 3 for each group. A Gene change levels based on RNA-Seq data. B The mRNA relative expression levels were determined by RT-qPCR. Student’s t test was employed to compare the differences between groups. The error bars represent mean values ± SDs. Statistical analysis results were visualized using GraphPad Prism5. *** indicates P < 0.001, ** indicates P < 0.01, * indicates P < 0.05