Fig. 6

Metabolome analysis of New Zealand white rabbit ovarian granulosa cells treated with DMSO and MLN4924. A PCA score plot of the metabolome. B OPLS-DA score plot of the metabolites in GCs. The red dots represent the DMSO group, while the blue squares represent the MLN4924 treatment group. C Volcano plot of differential metabolites. The x-axis represents the value of the log2-transformed fold change in metabolite abundance, while the y-axis represents the -log10-transformed P-values from the t test. Red dots represent DAMs with VIP ≥ 1 and P < 0.05, indicating upregulation; blue dots represent DAMs with VIP ≥ 1 and P < 0.05, indicating downregulation. The size of the dots is proportional to the VIP value of the metabolite. D The heatmap shows the relative expression of metabolites in the metabolic group. In the graph, each vertical column represents a sample, while each horizontal row represents a metabolite. The various colors indicate different levels of expression. E Significantly differential metabolisms were examined using KEGG pathway enrichment scatter plot statistical analysis. The x-axis represents the rich factor, the y-axis indicates different pathways, the color of the point is associated with the P-value, and the size is indicative of the enriched count